General description

Glucose is a primary energy source that naturally occurs in its free state in fruits and other plant parts. Abnormal glucose levels have been associated with several metabolic dysfunctions such as hypoglycemia, hyperglycemia, and diabetes mellitus. Measurements of glucose levels in tissues and body fluids (such as blood and urine) are often used for the diagnosis of glucose-related disorders. Glucose levels are also monitored to check the efficacy of therapeutics such as insulin and sulphonylureas in type 2 diabetics.

Principle

Glucose concentration is determined by a coupled enzyme assay, which results in a fluorometric (λ_ex = 535/λ_em = 587 nm) product proportional to the glucose present.

Suitability

High Sensitivity Glucose Assay Kit is a highly sensitive assay for determining glucose levels (ranging from 20-100pmole/well) in a variety of biological samples.